Sunita Subedi Paudel

Introduction

Welcome to my online portfolio.
I am a Ph.D candidate in the department of Physiology and Cell Biology along with Biomedical Engineering, Center of Lung Biology and Vascular Biology track at the University of South Alabama College of Medicine. I am schedule to graduate in May of 2023. I am working in the area of cell physiology and cell mechanics with
Dr. Dhananjay Tambe
and ,
Dr. Troy Stevens .

Education

University of South Alabama College of Medicine, Mobile, AL | Aug 2017 - Present
Ph. D. in Physiology, Cell Biology and Biomedical Engineering
Graduate Research Assistant
• GPA: 3.91 / 4.0
• Thesis: Effect of substrate stiffness on the lung endothelial phenotype
• Prepared polyacrylamide hydrogels of different stiffness and cultured endothelial cells from lungs and performed microscopic experiments over time to see morphological changes on endothelial cells and analyzed image for the quantitative measurement.
• Developed protocols for the 3D cell culture and calcium imaging on the endothelial monolayer.
• Trained junior graduate students and medical students all the techniques required to perform basic experiments including microscopy and image analysis.
• Developed software iTACS for the physiological properties of individual adherent cells with the use of ImageJ macro and Java (Co-first authored peer-reviewed scientific publication).
• Developed novel technique PFA for the quantification of local membrane dynamics of the endothelium using ImageJ macro code (First author peer-reviewed scientific publication).
• Performed microscopy experiments and analyzed data using Python and R programming (Co-authored on four peer-reviewed scientific publications).

University of Southern Mississippi, Hattiesburg, MS | Aug 2015 - Aug 2017
Master of Science in Computational Biophysics
Graduate Teaching and Research Assistant
• GPA: 3.97 / 4.0
• Thesis: Conformation of transmembrane segments of a protein by a coarse grain model
• Applied Monte-Carlo Simulations and FORTRAN and Python programming to study conformation change of the segment of protein chain based on the change in the entropy.
• Taught Astronomy (111 and 112) and Physics (101 and 201) lab for the undergraduate students with the use of simulation software.
• Trained students to use C++ program to solve the problems of Physics lab.
• Visualized data with the use of VMD, Python and MATLAB (Co-authored three peer-reviewed scientific publications).

Prithvi Narayan Campus Tribhuvan University, Nepal | December 2012
Bachelor of Science in Physics major and Chemistry minor
• Percentage: First Division (73.64%)

Shishu Niketan Higher Secondary School, Nepal | December 2008
+2 in Science (Physics, Chemistry, Biology and Mathematics)
• Percentage: Distinction (78.80%)

Karunanidhi Education Foundation Higher Secondary School, Nepal | December 2005
School Leaving Certificate (SLC)
• Percentage: Distinction (85.38%)

Morning Star Higher Secondary School, Nepal | December 2000
Elementary and Middle School Education
• Percentage: Distinction (82.90%)

Research

PEER-REVIEWED PUBLICATION

. Sunita Subedi Paudel, Althea deWeever, Sarah L. Sayner, Troy Stevens, Dhananjay Tambe, Subendothelial surface stiffness impairs migration and local inter-cellular membrane motion in pulmonary endothelium, American Journal of Physiology Lung Cell Physiology, 2022 (In Press).

. Reece Stevens, Mikhail Alexeyev, Natalya Kozhukhar, Viktoriya Pastukh, Sunita S. Paudel, Jessica Bell, Dhananjay Tambe, Troy Stevens, Ji Young Lee, Carbonic anhydrase IX proteoglycan-like and intracellular domains mediate pulmonary microvascular endothelial cell repair and angiogenesis, American Journal of Physiology Lung Cellular and Molecular Physiology; 323: L48–L57, 2022.

. Alyson Nguyen, Keith Battle, Sunita S. Paudel, Ningyoug Xu, Jessica Bell, Linn Ayers, Cassandra Chapman, Ajay Singh, Srinivas Palanki, Thomas Rich, Diego F. Alvarez, Troy Stevens, Dhananjay Tambe, Integrative Toolkit to Analyze Cellular Signals: Forces, Motion, Morphology, and Fluorescence, Journal of Visualized Experiments; 181, e63095, 2022.

. Reece P. Stevens, Sunita S. Paudel, Santina C. Johnson, Troy Stevens, Ji Young Lee, Endothelial metabolism in pulmonary vascular homeostasis and acute respiratory distress syndrome, American Journal of Physiology Lung Cellular and Molecular Physiology;321(2), L358-L376, 2021.

. Phoibe Renema, Natalya Kozhukhar, Viktoriya Pastukh, Domenico Spadafora, Sunita Subedi Paudel, Dhananjay T Tambe, Mikhail Alexeyev, Dara W Frank, Troy Stevens, Exoenzyme Y induces extracellular active caspase-7 accumulation independent from apoptosis: modulation of transmissible cytotoxicity, American Journal of Physiology Lung Cellular and Molecular Physiology; 319(2), 380-390, 2020.

. Ji Young Lee, Reece P. Stevens, Mary Kash, Chun Zhou, Anna Koloteva, Phoibe Renema, Sunita S Paudel, Troy Stevens, KD025 Shifts Pulmonary Endothelial Cell Bioenergetics and Decreases Baseline Lung Permeability, American Journal of Respiratory Cell and Molecular Biology; 63 (4), 519-530, 2020.

. Panisak Boonamnaj, Sunita Subedi Paudel, Warin Jetsadawisut, Sunan Kitjaruwankul, Pornthep Sompornpisut, R.B. Pandey, Thermal response of a protein (hHv1) by a coarse-grained MC and all-atom MD computer simulations, Physica A: Statistical Mechanics and its Applications 527:121310, 2019.

. Sunan Kitjaruwankul, Panisak Boonamnaj, Sunita S. Paudel, Warin Jetsadawisut, Pornthep Sompornpisut, Ras B.Pandey, Thermal-induced folding and unfolding of a transmembrane protein (CorA), Physica A: Statistical Mechanics and its Applications; 506 (987-992), 2018.

. Ras B. Pandey, Sunan Kitjaruwankul, Panisak Boonamnaj, Sunita S. Paudel, Warin Jetsadawisut, Pornthep Sompornpisut (2017). Conformational modulation of transmembrane segments of a protein (CorA) by effective media, International Journal of Virology & Infectious Diseases: 2 (1), 6, 2017.

PUBLISHED ABSTRACTS AND PRESENTATION
2022 - Dhananjay T. Tambe, Sunita Subedi Paudel, Jessica Bell, Thomas C. Rich, and Troy Stevens, An open-source toolkit to visualize cellular morphology, motion, fluorescence, and forces (2022), SPIE Photonics West.

2022 - Silas J. Leavesley, Santina Johnson, Sunita Paudel, Jennifer Knighten, Dhananjay Tambe, Michael Francis, Na Gong, Mark S. Taylor, and Thomas C. Rich, Combined hyperspectral imaging, monolayer stress microscopy, and S8 image analysis approaches for simultaneously interrogating cellular signals and biomechanics (2022), SPIE Photonics West.

2022 - Sunita Subedi Paudel, Navneet Singh, Katherine Cox-Flaherty, Mandy Pereira, Dhananjay T. Tambe, Troy Stevens, Elizabeth O. Harrington, and Corey E. Ventetuolo, Human pulmonary artery endothelial cells isolated from patients with pulmonary hypertension exhibit anoikis resistant (May 2022), American Thoracic Society.

2022 - Althea Deweever, Chun Choi, Victoria Pastukh, Meredith S. Gwin, Sarah B. Voth, Sunita Subedi Paudel, Allison Baumann, Natalya Kozhukhar, Trinity Elston, Ji Young Lee, Christopher Michael Francis, Silas Leavesley, Mikhail Alexeyev, Amy Nelson, Mike T Lin, Ron Balczon, and Troy Stevens, Autofluorescent detection of cytotoxic tau as a putative point of care method (May 2022), American Thoracic Society.

2022 - Sunita Subedi Paudel, Navneet Singh, Elizabeth O. Harrington, Corey E. Ventetuolo, Dhananjay T. Tambe, and Troy Stevens, Human pulmonary arterial endothelial cells from pulmonary arterial hypertension patients exhibit anoikis resistant (March 2022), Graduate Research Symposium, The University of South Alabama.

2021 - Sunita Subedi Paudel, Althea Deweever, Amy Nelson, Troy Stevens, and Dhananjay T. Tambe, Paracellular Fluctuations Analyzer - A Tool to Assess Rapid Paracellular Remodeling in Pulmonary Endothelium (May 2021), American Thoracic Society.

2021 - Althea Deweever, Sunita Subedi Paudel, Ron Balczon, and Troy Stevens, Untangling the Fluorescence Behaviour of Pulmonary Amyloids and Pseudomonas Aeruginosa’s Siderophore Pyoverdin (May 2021), American Thoracic Society.

2021 - Althea Deweever, Sunita Subedi Paudel, Silas Leavesely, Ron Balczon, and Troy Stevens, Iron Irreversibly Alters the Autofluorescence of Pulmonary Amyloids: Implications on Amyloid Detection in Clinical Samples (May 2021), American Thoracic Society.

2021 - Althea Deweever, Sunita Subedi Paudel, Ron Balczon, and Troy Stevens, Copper but not Zinc Ions Modify the Autofluorescence of Pathogenic and Antimicrobial Pulmonary Amyloids (May 2021), American Thoracic Society.

2021 - Alyson Nguyen, Keith Battle, Sunita Subedi Paudel, Jessica Bell, Linn Ayers, Thomas Rich, Diego Alvarez, Troy Stevens, and Dhanajay Tambe, An Automated In Vitro Experimental Platform to Analyze Structure, Motion and Forces in Adherent Cells (May 2021), FASEB Journal.

2021 - Althea deWeever, Meredith Gwin, Sarah Voth, Sunita Subedi Paudel, Silas Leavesely, Ron Balczon, and Troy Stevens, Development of a Novel Point Care of Care Test for Toxic Amyloids in Patients Recovering from Hospital Acquired Pneumonia (May 2021), FASEB Journal.

2021 - Sunita Subedi Paudel, Althea Deweever, Amy Nelson, Troy Stevens, and Dhananjay T. Tambe, Paracellular Fluctuations Analyzer - A Tool to Assess Rapid Paracellular Remodeling in Pulmonary Endothelium (March 2021), Graduate Research Symposium, The University of South Alabama.

2021 - Sunita Subedi Paudel, Althea deWeever, Amy Nelson, Troy Stevens, and Dhanajay Tambe, Effect of substrate stiffness on the morphological dynamics of pulmonary artery endothelial cells, (Jan 2021), Pulmonary Vascular Research Institute.

2020 - Sunita Subedi Paudel, Dhananjay Tambe, and Troy Stevens, Substrate Stiffness Acts as a Permeability-Inducing Mechanical Stimulus in Pulmonary Arterial Hypertension (May 2020) American Thoracic Society.

2020 - Meredith S. Gwin, Sarah Voth, Sunita Subedi Paudel, N. Onanyan, A. Darby, Christopher Michael Francis, and Troy Stevens, Essential Role for Gamma Secretase Activating Protein (GSAP) in Infection-Elicited Endothelial Proteinopathy, (May 2020) American Thoracic Society.

2020 - Sunita Subedi Paudel, Dhananjay Tambe, and Troy Stevens, Substrate Stiffness: A permeability-inducing mechanical stimulus in pulmonary endothelium, (March 2020) 36th Southern Biomedical Engineering Conference.

2019 - Sunita Subedi Paudel, Dhananjay Tambe, and Troy Stevens, How substrate stiffness is affecting endothelial cells? (March 2019), Graduate Research Symposium, The University of South Alabama.

2018 - Sunita Subedi Paudel, Troy Stevens, and Dhananjay Tambe, Contribution of Endothelial Substrate Stiffness to Activation of Store-Operated Calcium Entry: Impact on Permeability, (Nov 2018), COM Research Conference, University of South Alabama- College of Medicine.

2018 - Warin Jetsadawisut, Sunan Kitjaruwankul, Panisak Boonamnaj, Sunita Subedi Paudel, Pornthep Sompornpisut, and Ras Pandey, Conformational response of a membrane protein (CorA) by a coarse-grained model, (Mar 2018), APS March Meeting.

2017 - Sunita Subedi Paudel, and Ras B. Pandey, Conflicting thermal responses of the cytoplasmic domain of hHv1 channel by all-atom MD and all-residue coarse-grained MC simulations, (April 2017), Susan A. Siltanen Graduate Student Research Symposium, The University of Southern Mississippi.

2017 - Panisak Boonamnaj, Sunita Subedi Paudel, Warin Jetsadawisut, Sunan Kitjaruwankul, Pornthep Sompornpisut, and Ras Pandey, Conflicting thermal response of a monomer and a tandem dimer of a membrane protein segment (hHv1), (Mar 2017), APS March Meeting.

2017 - Sunita Subedi Paudel, and Ras B. Pandey, Effect of temperature on the structure of a protein segment (hHv1) by a coarse-grained model (Feb 2017), Mississippi Academy of Science 81st Annual Conference, The University of Southern Mississippi.

ORAL PRESENTATIONS
2017 - Present - Presentations at Center for Lung Biology Research in Progress once a year, University of South Alabama.

2022 - “Substrate Stiffness: A mechanical determinant of endothelial phenotype.” National Jewish Health Seminar.

2022 - “Human Pulmonary Artery Endothelial Cells Isolated from Patients with Pulmonary Hypertension Exhibit Anoikis Resistance.” VuMedi (Virtual Presentation) ATS 2022

2022 - “Human pulmonary artery endothelial cells isolated from patients with pulmonary hypertension can survive without microenvironment.” Frontiers of Engineering, Automation and Technology Seminars, The University of South Alabama.

2020 - “Substrate stiffness: A permeability inducing mechanical stimulus in pulmonary endothelium.” Southern Biomedical Engineering Society.

2017 - “Effect of temperature on the structure of a protein segment (hHv1) by a coarse-grained model.” Mississippi Academy of Science.

2017 - “Conflicting thermal response of a monomer and a tandem dimer of a membrane protein segment (hHv1).” American Physical Society.

2017 - “Contradictory thermal responses of the cytoplasmic domain of hHv1 channel by all-atom MD and all-residue coarse-grained MC simulations.” Susan A. Siltanen Graduate Student Research Symposium, The University of Southern Mississippi.

Professional Experience

EXPERIENCES

2017 - Present Graduate Research Assistant, taught graduate, medical and undergraduate students various lab techniques, The University of South Alabama, Mobile, AL.

2015 - 2017 Graduate Teaching Assistant, taught undergraduate students Astronomy (ASL111 and ASL 112) and Physics (PHY 101 and PHY 201). The University of Southern Mississippi, Hattiesburg, MS.

2012 - 2013 Department Head of Science, taught overall science to high school students that include Physics, Chemistry and Biology, Shining Star Secondary School, Pokhara, Nepal.

PROFESSIONAL SOCIETY MEMBERSHIPS

2020 – Present | Pulmonary Vascular Research Institution

2019 – Present | American Heart Association

2019 – Present | Southern Biomedical Engineering Society

2017 – Present | American Thoracic Society

2015 – 2018 | American Physical Society

LEADERSHIPS

2020-Present | University of South Alabama College of Medicine Senator

2020-Present | University of South Alabama Basic Medical Science Student Advisory Committee

2020-2021 | University of South Alabama Basic Medical Science GradPal

2020-2021 | University of South Alabama Justice Initiative Student Advisor Team

2019-2021 | University of South Alabama International Advisory Committee Leader

2015-2017 | University of Southern Mississippi Student Physical Society Treasurer

Contact

• Twitter
• LinkedIn
• Facebook
• Instagram
• GitHub
• ResearchGate

Elements

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